By American Academy of Ophthalmology, Robert H. Rosa Jr. MD
Part four provides fabrics in elements: half I, Ophthalmic Pathology; and half II, Intraocular Tumors: medical points. half I makes use of a hierarchy that strikes from normal to precise to aid derive a differential analysis for a selected tissue. half II is a compilation of chosen medical points of significance to the overall ophthalmologist. Following half II are the yankee Joint Committee on melanoma 2010 staging types for ocular and adnexal tumors.
Upon of entirety of part four, readers could be capable to:
Describe a dependent method of figuring out significant ocular stipulations in accordance with a hierarchical framework of topography, affliction method, normal analysis and differential diagnosis
Summarize the stairs in dealing with ocular specimens for pathologic learn, together with acquiring, dissecting, processing, and marking tissues
Identify these ophthalmic lesions that point out systemic affliction and are possibly existence threatening
Read or Download 2014-2015 Basic and Clinical Science Course (BCSC): Section 4: Ophthalmic Pathology and Intraocular Tumors PDF
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Additional resources for 2014-2015 Basic and Clinical Science Course (BCSC): Section 4: Ophthalmic Pathology and Intraocular Tumors
Terp1e a l~g,~ ll1 . d formalin diffuses at a rate of approximately 1 mm/hr; therefore, globes . drunetei, an . u, ' b fixed at ]east 12 hoLu·s prior to processing. It is generally desirable to suspend an sho~ddt". ately 10:1 for at least 24 hours prior to processing e 111 1onna eY· . dequate fixation. Differen institutions may use different protocols, and preop- to en ur ea ' . . e consultation 1s cntical. era t ,v ns >n. ild '0 ,rs as or Tissue Processing The infiltration and embedding process removes most of the water from the tissue and relaces the water with paraffin.
J 1 1• I ~' I IJU I, l if : • '· ( . a1;11w1 1ab . ·o n,ity 1 Figure 3-4 The section of a melanoma at the far left is colorless except for mild indigenous pigmentation in the tissue . Moving to the right, note the slides sta ined w ith hematoxylin only, eosin only, and both hematoxylin and eosin. /Courtes y of Hans E. J II CHAPTER 3: ~ re of in m rable 3· 2 c ommon Stains Used in Ophthalmic Pathology lin and eosin (H&E) . d·,c acid-Schiff (PAS) peno ,la, Alcian blue 0 a Aliza ri n red Colloidal iron td IS V, Qllaterial Stained: Color Example Nucleus: blue Cytoplasm: red Glycogen and proteoglycans: magenta Acid mucopolysaccharide: blue Calcium: red Acid mucopolysaccharide: blue Amyloid: orange, red-green dichroism Acid-fast organisms: red Fungal elements: black General tissue stain (Fig 3-2E) Descemet membrane (Fig 6-17E) Cavernous optic atrophy (Fig 15-10B) Band keratopathy Macular dystrophy (Fig 6-21C) Lattice dystrophy (Fig 6-23C, D) Atypical mycobacterium Fusarium (Fig 6-7B) Amyloid: purple, violet Bacteria positive: blue Bacteria negative: red Lattice dystrophy Bacterial infection Collagen: blue Granular dystrophy (Fig 6-22C) Red deposits Fleischer ring Lattice dystrophy Temporal artery elastic layer (Fig 15-6B) Band keratopathy (Fig 6-12C) Stain Hema t o Xy Specimen Handling • 31 Congo red Ziehl-Neelsen Gornori methenamine silver (GMS) Crystal violet Gram stain (tissue Brown & Brenn [B&Bl or Brown & Hopps [B&H] stain) Masson trichrome Perls Prussian blue Thioflavin T (ThT) Verhoeff-van Gieson (elastic stain) von Kassa Muscle: red Iron: blue Amyloid: fluorescent yellow Elastic fibers: black Calcium phosphate salts: black ..
Commercial microarray and PCR platforms now exist that can be used for assigning biopsy-sized tumor samples to 1 of 2 distinct molecular classes, based on gene expression analysis that distinguishes low-grade tumors from high-grade tumors. The selection of commercially available microarray and PCR kits is growing rapidly. Leukemias, lymphomas, and soft-tissue tumors represent a heterogeneous group of lesions whose classification continues to evolve as a result of advances in cytogenetic and molecular techniques.